One-step triplex PCR/RT-PCR to detect canine distemper virus, canine parvovirus and canine kobuvirus
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One-step triplex PCR/RT-PCR to detect canine distemper virus, canine parvovirus, and canine kobuvirus.
To rapidly distinguish Canine distemper virus (CDV), canine parvovirus (CPV), and canine kobuvirus (CaKoV) in practice, a one-step multiplex PCR/RT-PCR assay was developed, with detection limits of 102.1 TCID50 for CDV, 101.9 TCID50 for CPV and 103 copies for CaKoV. This method did not amplify nonspecific DNA or RNA from other canine viruses. Therefore, the assay provides a sensitive tool for t...
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ژورنال
عنوان ژورنال: Journal of Veterinary Medical Science
سال: 2019
ISSN: 0916-7250,1347-7439
DOI: 10.1292/jvms.17-0442